› 4grr

X-ray diffraction
1.47Å resolution

characterization of N- and C- terminus mutants of human MIF

Released:
DOI: 10.2210/pdb4grr/pdb
PDB entry 4grr coloured by chain, front view.
PDB entry 4grr coloured by chain, side view.
PDB entry 4grr coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Fan C, Lolis E

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 116 amino acids
Theoretical weight: 12.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14174 (Residues: 3-115; Coverage: 98%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand CL 11 x CL
Ligand AVR 1 x AVR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 95.546Å b: 95.546Å c: 103.879Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.149 0.172
Expression system: Escherichia coli BL21(DE3)

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