› 4gs0

X-ray diffraction
1.8Å resolution

Crystal structure of SHP1 catalytic domain with JAK1 activation loop peptide

Released:
DOI: 10.2210/pdb4gs0/pdb
PDB entry 4gs0 coloured by chain, front view.
PDB entry 4gs0 coloured by chain, side view.
PDB entry 4gs0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-guided studies of the SHP-1/JAK1 interaction provide new insights into phosphatase catalytic domain substrate recognition.
Alicea-Velázquez NL, Jakoncic J, Boggon TJ
J Struct Biol 181 243-51 (2013)
PMID: 23296072

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151413 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 35.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29350 (Residues: 243-528; Coverage: 48%)
Gene names: HCP, PTP1C, PTPN6
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
Tyrosine-protein kinase JAK1 Chain: C
Molecule details ›
Chain: C
Length: 4 amino acids
Theoretical weight: 550 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand FTY 1 x FTY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P31
Unit cell:
a: 43.935Å b: 43.935Å c: 258.112Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.162 0.195
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

The molecular regulation of Janus kinase (JAK) activation.
Babon et al. (2014)
2 more

2 mentions without citation

Integrated bioinformatic analysis reveals the underlying molecular mechanism of and potential drugs for pulmonary arterial hypertension.
Dong et al. (2021)
1 more