› 4gum

X-ray diffraction
2.33Å resolution

Cystal structure of locked-trimer of human MIF

Released:
DOI: 10.2210/pdb4gum/pdb
PDB entry 4gum coloured by chain, front view.
PDB entry 4gum coloured by chain, side view.
PDB entry 4gum coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
MIF intersubunit disulfide mutant antagonist supports activation of CD74 by endogenous MIF trimer at physiologic concentrations.
Fan C, Rajasekaran D, Syed MA, Leng L, Loria JP, Bhandari V, Bucala R, Lolis EJ
Proc Natl Acad Sci U S A 110 10994-9 (2013)
PMID: 23776208

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 114 amino acids
Theoretical weight: 12.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6DN04 (Residues: 2-115; Coverage: 99%)
Gene name: MIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand CL 8 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 50.315Å b: 100.381Å c: 199.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

MIF, a controversial cytokine: a review of structural features, challenges, and opportunities for drug development.
Bloom et al. (2016)
4 more

1 mention without citation

MIF intersubunit disulfide mutant antagonist supports activation of CD74 by endogenous MIF trimer at physiologic concentrations.
Fan et al. (2013)