› 4guo

X-ray diffraction
3.19Å resolution

structure of p73 DNA binding domain complex with 12 bp DNA

Released:
DOI: 10.2210/pdb4guo/pdb
PDB entry 4guo coloured by chain, front view.
PDB entry 4guo coloured by chain, side view.
PDB entry 4guo coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ethayathulla AS, Viadiu H

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117561 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Tumor protein p73 Chains: A, B, C, D, I, J, K, L
Molecule details ›
Chains: A, B, C, D, I, J, K, L
Length: 210 amino acids
Theoretical weight: 23.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15350 (Residues: 115-312; Coverage: 31%)
Gene names: P73, TP73
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA (5'-D(*CP*GP*GP*GP*CP*AP*AP*GP*CP*CP*CP*G)-3') Chains: E, G, M, O
Molecule details ›
Chains: E, G, M, O
Length: 12 nucleotides
Theoretical weight: 3.67 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*GP*GP*GP*CP*TP*TP*GP*CP*CP*CP*G)-3') Chains: F, H, N, P
Molecule details ›
Chains: F, H, N, P
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 82.25Å b: 104.329Å c: 123.324Å
α: 90° β: 96.38° γ: 90°
R-values:
R R work R free
0.211 0.208 0.27
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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