› 4gwc

X-ray diffraction
1.9Å resolution

Crystal Structure of Mn2+2,Zn2+-Human Arginase I

Released:
DOI: 10.2210/pdb4gwc/pdb
PDB entry 4gwc coloured by chain, front view.
PDB entry 4gwc coloured by chain, side view.
PDB entry 4gwc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of non-native metal clusters in human arginase I.
D'Antonio EL, Hai Y, Christianson DW
Biochemistry 51 8399-409 (2012)
PMID: 23061982

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 34.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05089 (Residues: 1-322; Coverage: 100%)
Gene name: ARG1
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3
Unit cell:
a: 90.829Å b: 90.829Å c: 70.068Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.154 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Manganese Is Essential for Neuronal Health.
Horning et al. (2015)
4 more

1 mention without citation

Structure and function of non-native metal clusters in human arginase I.
D'Antonio et al. (2012)