› 4gzl

X-ray diffraction
2Å resolution

Crystal structure of RAC1 Q61L mutant

Released:
DOI: 10.2210/pdb4gzl/pdb
PDB entry 4gzl coloured by chain, front view.
PDB entry 4gzl coloured by chain, side view.
PDB entry 4gzl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
RAC1P29S is a spontaneously activating cancer-associated GTPase.
Davis MJ, Ha BH, Holman EC, Halaban R, Schlessinger J, Boggon TJ
Proc Natl Acad Sci U S A 110 912-7 (2013)
PMID: 23284172

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63000 (Residues: 2-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 49.83Å b: 72.94Å c: 91.597Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

33 review citations

Rho GTPases modulate malignant transformation of tumor cells.
Orgaz et al. (2014)
32 more

5 mentions without citation

RAC1 as a Therapeutic Target in Malignant Melanoma.
Cannon et al. (2020)
4 more