› 4gzm

X-ray diffraction
2.8Å resolution

Crystal structure of RAC1 F28L mutant

Released:
DOI: 10.2210/pdb4gzm/pdb
PDB entry 4gzm coloured by chain, front view.
PDB entry 4gzm coloured by chain, side view.
PDB entry 4gzm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
RAC1P29S is a spontaneously activating cancer-associated GTPase.
Davis MJ, Ha BH, Holman EC, Halaban R, Schlessinger J, Boggon TJ
Proc Natl Acad Sci U S A 110 912-7 (2013)
PMID: 23284172

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 22.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63000 (Residues: 2-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GSP 2 x GSP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 40.094Å b: 98.807Å c: 51.994Å
α: 90° β: 90.21° γ: 90°
R-values:
R R work R free
0.255 0.254 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

33 review citations

Rho GTPases modulate malignant transformation of tumor cells.
Orgaz et al. (2014)
32 more

3 mentions without citation

RAC1P29S is a spontaneously activating cancer-associated GTPase.
Davis et al. (2013)
2 more