› 4h1l

X-ray diffraction
3.3Å resolution

TCR interaction with peptide mimics of nickel offers structural insights in nickel contact allergy

Released:
DOI: 10.2210/pdb4h1l/pdb
PDB entry 4h1l coloured by chain, front view.
PDB entry 4h1l coloured by chain, side view.
PDB entry 4h1l coloured by chain, top view.
Source organisms:
Primary publication:
T-cell receptor (TCR) interaction with peptides that mimic nickel offers insight into nickel contact allergy.
Yin L, Crawford F, Marrack P, Kappler JW, Dai S
Proc Natl Acad Sci U S A 109 18517-22 (2012)
PMID: 23091041

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-125807 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 178 amino acids
Theoretical weight: 20.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01903 (Residues: 28-205; Coverage: 78%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DR beta 3 chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 187 amino acids
Theoretical weight: 21.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P79483 (Residues: 35-217; Coverage: 77%)
Gene name: HLA-DRB3
Sequence domains:
Structure domains:
mimotope peptide Chains: C, F
Molecule details ›
Chains: C, F
Length: 13 amino acids
Theoretical weight: 1.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Ig-like domain-containing protein Chains: G, I
Molecule details ›
Chains: G, I
Length: 113 amino acids
Theoretical weight: 12.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: L7MTK9 (Residues: 1-113; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: H, J
Molecule details ›
Chains: H, J
Length: 111 amino acids
Theoretical weight: 12.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: L7MTL0 (Residues: 1-111; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P63
Unit cell:
a: 186.722Å b: 186.722Å c: 166.702Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.264 0.263 0.285
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

5 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
4 more

10 mentions without citation

Coevolution of T-cell receptors with MHC and non-MHC ligands.
Castro et al. (2015)
9 more