› 4h5r

X-ray diffraction
1.64Å resolution

HSC70 NBD with Na, Cl and glycerol

Released:
DOI: 10.2210/pdb4h5r/pdb
PDB entry 4h5r coloured by chain, front view.
PDB entry 4h5r coloured by chain, side view.
PDB entry 4h5r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
New crystal structures of HSC-70 ATP binding domain confirm the role of individual binding pockets and suggest a new method of inhibition.
Zhang Z, Cellitti J, Teriete P, Pellecchia M, Stec B
Biochimie 108 186-92 (2015)
PMID: 25433210

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-145675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock cognate 71 kDa protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 42.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P11142 (Residues: 2-384; Coverage: 59%)
Gene names: HSC70, HSP73, HSPA10, HSPA8
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NA 2 x NA
Ligand PO4 7 x PO4
Ligand CL 2 x CL
Ligand SO4 3 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: C2
Unit cell:
a: 138.637Å b: 102.462Å c: 77.776Å
α: 90° β: 119.8° γ: 90°
R-values:
R R work R free
0.207 0.205 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

HSPA8/HSC70 in Immune Disorders: A Molecular Rheostat that Adjusts Chaperone-Mediated Autophagy Substrates.
Bonam et al. (2019)
5 other articles

2 mentions without citation

Disrupted Hydrogen-Bond Network and Impaired ATPase Activity in an Hsc70 Cysteine Mutant.
O'Donnell et al. (2018)
1 more