› 4h71

X-ray diffraction
1.93Å resolution

Human Plk1-PBD in complex with Poloxime ((E)-4-(hydroxyimino)-2-isopropyl-5-methylcyclohexa-2,5-dienone)

Released:
DOI: 10.2210/pdb4h71/pdb
PDB entry 4h71 coloured by chain, front view.
PDB entry 4h71 coloured by chain, side view.
PDB entry 4h71 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Thymoquinone blocks pSer/pThr recognition by Plk1 Polo-box domain as a phosphate mimic.
Yin Z, Song Y, Rehse PH
ACS Chem Biol 8 303-8 (2013)
PMID: 23135290

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156752 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 27.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 367-603; Coverage: 39%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PXE 1 x PXE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 33.26Å b: 102.75Å c: 68.49Å
α: 90° β: 93.85° γ: 90°
R-values:
R R work R free
0.191 0.188 0.237
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Understanding the Polo Kinase machine.
Archambault et al. (2015)
6 more

3 mentions without citation

Recent Advances and New Strategies in Targeting Plk1 for Anticancer Therapy.
Lee et al. (2015)
2 more