› 4h8w

X-ray diffraction
1.85Å resolution

Crystal structure of non-neutralizing and ADCC-potent antibody N5-i5 in complex with HIV-1 clade A/E gp120 and sCD4.

Released:
DOI: 10.2210/pdb4h8w/pdb
PDB entry 4h8w coloured by chain, front view.
PDB entry 4h8w coloured by chain, side view.
PDB entry 4h8w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural definition of an antibody-dependent cellular cytotoxicity response implicated in reduced risk for HIV-1 infection.
Acharya P, Tolbert WD, Gohain N, Wu X, Yu L, Liu T, Huang W, Huang CC, Kwon YD, Louder RK, Luongo TS, McLellan JS, Pancera M, Yang Y, Zhang B, Flinko R, Foulke JS, Sajadi MM, Kamin-Lewis R, Robinson JE, Martin L, Kwong PD, Guan Y, DeVico AL, Lewis GK, Pazgier M
J Virol 88 12895-906 (2014)
PMID: 25165110

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208500 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 353 amino acids
Theoretical weight: 39.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q0ED31 (Residues: 43-486; Coverage: 41%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
FAB HEAVY CHAIN OF ADCC AND NON-NEUTRALIZING ANTI-HIV-1 ANTIBODY N5-I5 Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 23.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
FAB LIGHT CHAIN OF ADCC AND NON-NEUTRALIZING ANTI-HIV-1 ANTIBODY N5-I5 Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 22.94 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
T-cell surface glycoprotein CD4 Chain: C
Molecule details ›
Chain: C
Length: 185 amino acids
Theoretical weight: 20.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01730 (Residues: 26-208; Coverage: 42%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand NAG 10 x NAG
Ligand MRD 3 x MRD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 92.251Å b: 103.909Å c: 134.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.215
Expression system: Homo sapiens

Quick links

Citations

13 review citations

HIV antibodies for treatment of HIV infection.
Margolis et al. (2017)
12 more

14 mentions without citation

Conformational Masking and Receptor-Dependent Unmasking of Highly Conserved Env Epitopes Recognized by Non-Neutralizing Antibodies That Mediate Potent ADCC against HIV-1.
Lewis et al. (2015)
13 more