› 4hc7

X-ray diffraction
2.65Å resolution

Crystal structure of the full DNA binding domain of GATA3-complex 2

Released:
DOI: 10.2210/pdb4hc7/pdb
PDB entry 4hc7 coloured by chain, front view.
PDB entry 4hc7 coloured by chain, side view.
PDB entry 4hc7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA binding by GATA transcription factor suggests mechanisms of DNA looping and long-range gene regulation.
Chen Y, Bates DL, Dey R, Chen PH, Machado AC, Laird-Offringa IA, Rohs R, Chen L
Cell Rep 2 1197-206 (2012)
PMID: 23142663

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Trans-acting T-cell-specific transcription factor GATA-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 12.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23771 (Residues: 260-370; Coverage: 25%)
Gene name: GATA3
Sequence domains: GATA zinc finger
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*TP*TP*CP*CP*TP*AP*AP*AP*TP*CP*AP*GP*AP*GP*AP*TP*AP*AP*CP*C)-3') Chains: W, Y
Molecule details ›
Chains: W, Y
Length: 20 nucleotides
Theoretical weight: 6.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*AP*GP*GP*TP*TP*AP*TP*CP*TP*CP*TP*GP*AP*TP*TP*TP*AP*GP*G)-3') Chains: X, Z
Molecule details ›
Chains: X, Z
Length: 20 nucleotides
Theoretical weight: 6.18 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 65.765Å b: 65.373Å c: 72.922Å
α: 90° β: 96.75° γ: 90°
R-values:
R R work R free
0.226 0.226 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

Absence of a simple code: how transcription factors read the genome.
Slattery et al. (2014)
11 more

8 mentions without citation

Targeting GATA4 for cardiac repair.
Välimäki et al. (2020)
7 more