› 4hdo

X-ray diffraction
1.67Å resolution

Crystal structure of the binary Complex of KRIT1 bound to the Rap1 GTPase

Released:
DOI: 10.2210/pdb4hdo/pdb
PDB entry 4hdo coloured by chain, front view.
PDB entry 4hdo coloured by chain, side view.
PDB entry 4hdo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the ternary complex of Krev interaction trapped 1 (KRIT1) bound to both the Rap1 GTPase and the heart of glass (HEG1) cytoplasmic tail.
Gingras AR, Puzon-McLaughlin W, Ginsberg MH
J Biol Chem 288 23639-49 (2013)
PMID: 23814056

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126334 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Krev interaction trapped protein 1 Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 37.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00522 (Residues: 417-736; Coverage: 44%)
Gene names: CCM1, KRIT1
Sequence domains: FERM central domain
Structure domains:
Ras-related protein Rap-1b Chain: B
Molecule details ›
Chain: B
Length: 167 amino acids
Theoretical weight: 19.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61224 (Residues: 1-167; Coverage: 91%)
Gene names: OK/SW-cl.11, RAP1B
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 57.85Å b: 77.75Å c: 58.85Å
α: 90° β: 91.2° γ: 90°
R-values:
R R work R free
0.213 0.212 0.231
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Cerebral cavernous malformation proteins at a glance.
Draheim et al. (2014)
10 more

6 mentions without citation

The structure of the ternary complex of Krev interaction trapped 1 (KRIT1) bound to both the Rap1 GTPase and the heart of glass (HEG1) cytoplasmic tail.
Gingras et al. (2013)
5 more