› 4hez

X-ray diffraction
1.34Å resolution

Activity Enhancers of H64A Variant of Human Carbonic Anhydrase II Possess Multiple Binding Sites within and around the Enzyme Structure

Released:
DOI: 10.2210/pdb4hez/pdb
PDB entry 4hez coloured by chain, front view.
PDB entry 4hez coloured by chain, side view.
PDB entry 4hez coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into activity enhancement and inhibition of H64A carbonic anhydrase II by imidazoles.
Aggarwal M, Kondeti B, Tu C, Maupin CM, Silverman DN, McKenna R
IUCrJ 1 129-35 (2014)
PMID: 25075329

Function and Biology Details

Reactions catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Urea = cyanamide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00918 (Residues: 1-260; Coverage: 100%)
Gene name: CA2
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand 1MZ 7 x 1MZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 42.291Å b: 41.607Å c: 71.866Å
α: 90° β: 104.46° γ: 90°
R-values:
R R work R free
0.152 0.151 0.17
Expression system: Escherichia coli

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Citations

9 review citations

Carbonic anhydrase activators.
Supuran CT. (2018)
8 more

1 mention without citation

Structural insight into activity enhancement and inhibition of H64A carbonic anhydrase II by imidazoles.
Aggarwal et al. (2014)