› 4hi8

X-ray diffraction
1.2Å resolution

Structure of integrin-linked kinase ankyrin repeat domain in complex with PINCH1 LIM1 domain collected at high energy, wavelength 0.32800

Released:
DOI: 10.2210/pdb4hi8/pdb
PDB entry 4hi8 coloured by chain, front view.
PDB entry 4hi8 coloured by chain, side view.
PDB entry 4hi8 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Stiegler AL, Jakoncic J, Stojanoff V, Chiswell BP, Calderwood DA, Boggon TJ

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155700 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Integrin-linked protein kinase Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13418 (Residues: 1-174; Coverage: 39%)
Gene names: ILK, ILK1, ILK2
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain
LIM and senescent cell antigen-like-containing domain protein 1 Chain: B
Molecule details ›
Chain: B
Length: 72 amino acids
Theoretical weight: 8.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48059 (Residues: 6-68; Coverage: 19%)
Gene names: LIMS1, PINCH, PINCH1
Sequence domains: LIM domain
Structure domains: Cysteine Rich Protein

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P1
Unit cell:
a: 37.093Å b: 41.859Å c: 46.983Å
α: 79.87° β: 68.82° γ: 85.77°
R-values:
R R work R free
0.145 0.144 0.168
Expression system: Escherichia coli

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