› 4hl4

X-ray diffraction
2.2Å resolution

Crystal structure of the human TBC1D20 RabGAP domain

Released:
DOI: 10.2210/pdb4hl4/pdb
PDB entry 4hl4 coloured by chain, front view.
PDB entry 4hl4 coloured by chain, side view.
PDB entry 4hl4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Catalytic mechanism of a mammalian Rab·RabGAP complex in atomic detail.
Gavriljuk K, Gazdag EM, Itzen A, Kötting C, Goody RS, Gerwert K
Proc Natl Acad Sci U S A 109 21348-53 (2012)
PMID: 23236136

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TBC1 domain family member 20 Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BZ9 (Residues: 14-305; Coverage: 73%)
Gene names: C20orf140, TBC1D20
Sequence domains: Rab-GTPase-TBC domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BEF 1 x BEF
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 73.55Å b: 73.55Å c: 113Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.203 0.235
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Rab GTPase regulation of membrane identity.
Pfeffer SR. (2013)
7 more

3 mentions without citation

Catalytic mechanism of a mammalian Rab·RabGAP complex in atomic detail.
Gavriljuk et al. (2012)
2 more