› 4hna

X-ray diffraction
3.19Å resolution

Kinesin motor domain in the ADP-MG-ALFX state in complex with tubulin and a DARPIN

Released:
DOI: 10.2210/pdb4hna/pdb
PDB entry 4hna coloured by chain, front view.
PDB entry 4hna coloured by chain, side view.
PDB entry 4hna coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a kinesin-tubulin complex and implications for kinesin motility.
Gigant B, Wang W, Dreier B, Jiang Q, Pecqueur L, Plückthun A, Wang C, Knossow M
Nat Struct Mol Biol 20 1001-7 (2013)
PMID: 23872990

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-112160 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.98 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
DESIGNED ANKYRIN REPEAT PROTEIN (DARPIN) D2 Chain: D
Molecule details ›
Chain: D
Length: 169 amino acids
Theoretical weight: 18.14 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain
Kinesin-1 heavy chain Chain: K
Molecule details ›
Chain: K
Length: 349 amino acids
Theoretical weight: 39.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33176 (Residues: 1-349; Coverage: 36%)
Gene names: KIF5B, KNS, KNS1
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 3 x MG
Ligand GDP 1 x GDP
Ligand ALF 2 x ALF
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 77.05Å b: 160.54Å c: 174.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.211
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
12 more

48 mentions without citation

The contribution of αβ-tubulin curvature to microtubule dynamics.
Brouhard et al. (2014)
47 more