› 4hwb

X-ray diffraction
2.61Å resolution

Crystal structure of ectodomain 3 of the IL-13 receptor alpha 1 in complex with a human neutralizing monoclonal antibody fragment

Released:
DOI: 10.2210/pdb4hwb/pdb
PDB entry 4hwb coloured by chain, front view.
PDB entry 4hwb coloured by chain, side view.
PDB entry 4hwb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Production of a human neutralizing monoclonal antibody and its crystal structure in complex with ectodomain 3 of the interleukin-13 receptor α1.
Redpath NT, Xu Y, Wilson NJ, Fabri LJ, Baca M, Andrews AE, Braley H, Lu P, Ireland C, Ernst RE, Woods A, Forrest G, An Z, Zaller DM, Strohl WR, Luo CS, Czabotar PE, Garrett TP, Hilton DJ, Nash AD, Zhang JG, Nicola NA
Biochem J 451 165-75 (2013)
PMID: 23384096

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211527 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-13 receptor subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 14.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P78552 (Residues: 223-336; Coverage: 28%)
Gene names: IL13R, IL13RA, IL13RA1
Structure domains: Immunoglobulins
Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 227 amino acids
Theoretical weight: 24.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand NHE 5 x NHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P4122
Unit cell:
a: 123.48Å b: 123.48Å c: 181.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.224
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Toll-Like Receptor 3 Signal in Dendritic Cells Benefits Cancer Immunotherapy.
Matsumoto et al. (2017)
5 other articles

4 mentions without citation

"Redirecting an anti-IL-1β antibody to bind a new, unrelated and computationally predicted epitope on hIL-17A".
Fischman et al. (2023)
3 more