› 4hzh

X-ray diffraction
3.3Å resolution

Structure of recombinant Gla-domainless prothrombin mutant S525A

Released:
DOI: 10.2210/pdb4hzh/pdb
PDB entry 4hzh coloured by chain, front view.
PDB entry 4hzh coloured by chain, side view.
PDB entry 4hzh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of prothrombin reveals conformational flexibility and mechanism of activation.
Pozzi N, Chen Z, Gohara DW, Niu W, Heyduk T, Di Cera E
J Biol Chem 288 22734-44 (2013)
PMID: 23775088

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prothrombin Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 60.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 90-622; Coverage: 89%)
Gene name: F2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 82.584Å b: 103.136Å c: 149.549Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.296 0.295 0.329
Expression system: Homo sapiens

Quick links

Citations

6 review citations

Stable mammalian producer cell lines for structural biology.
Büssow K. (2015)
5 more

6 mentions without citation

Crystal structure of prothrombin reveals conformational flexibility and mechanism of activation.
Pozzi et al. (2013)
5 more