› 4i0c

X-ray diffraction
1.95Å resolution

The structure of the camelid antibody cAbHuL5 in complex with human lysozyme

Released:
DOI: 10.2210/pdb4i0c/pdb
PDB entry 4i0c coloured by chain, front view.
PDB entry 4i0c coloured by chain, side view.
PDB entry 4i0c coloured by chain, top view.
Source organisms:
Primary publication:
A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.
De Genst E, Chan PH, Pardon E, Hsu SD, Kumita JR, Christodoulou J, Menzer L, Chirgadze DY, Robinson CV, Muyldermans S, Matagne A, Wyns L, Dobson CM, Dumoulin M
J Phys Chem B 117 13245-13258 (2013)
PMID: 23919586

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211422 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysozyme C Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.72 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P61626 (Residues: 19-148; Coverage: 100%)
Gene names: LYZ, LZM
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme
cAbHuL5 antibody Chains: C, D
Molecule details ›
Chains: C, D
Length: 132 amino acids
Theoretical weight: 14.6 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CL 7 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P43212
Unit cell:
a: 96.321Å b: 96.321Å c: 156.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.219
Expression systems:
  • Komagataella pastoris
  • Escherichia coli

Quick links

Citations

6 review citations

Camelid nanobodies: killing two birds with one stone.
Desmyter et al. (2015)
5 more

4 mentions without citation

Five of Five VHHs Neutralizing Poliovirus Bind the Receptor-Binding Site.
Strauss et al. (2016)
3 more