› 4i18

X-ray diffraction
3.24Å resolution

Crystal structure of human prolactin receptor complexed with Fab fragment

Released:
DOI: 10.2210/pdb4i18/pdb
PDB entry 4i18 coloured by chain, front view.
PDB entry 4i18 coloured by chain, side view.
PDB entry 4i18 coloured by chain, top view.
Source organisms:
Primary publication:
Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling.
Rizk SS, Kouadio JL, Szymborska A, Duguid EM, Mukherjee S, Zheng J, Clevenger CV, Kossiakoff AA
Cell Commun Signal 13 1 (2015)
PMID: 25589173

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210695 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
antibody light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 217 amino acids
Theoretical weight: 23.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 236 amino acids
Theoretical weight: 25.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Prolactin receptor Chains: C, R
Molecule details ›
Chains: C, R
Length: 211 amino acids
Theoretical weight: 24.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16471 (Residues: 25-235; Coverage: 35%)
Gene name: PRLR
Sequence domains: Erythropoietin receptor, ligand binding
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand GOL 4 x GOL
Ligand CA 2 x CA
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P61
Unit cell:
a: 285.826Å b: 285.826Å c: 62.248Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.191 0.246
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Allosteric Modulation as a Unifying Mechanism for Receptor Function and Regulation.
Changeux et al. (2016)
4 more

3 mentions without citation

Structural model for phenylalkylamine binding to L-type calcium channels.
Cheng et al. (2009)
2 more