› 4i2x

X-ray diffraction
2.48Å resolution

Crystal structure of Signal Regulatory Protein gamma (SIRP-gamma) in complex with FabOX117

Released:
DOI: 10.2210/pdb4i2x/pdb
PDB entry 4i2x coloured by chain, front view.
PDB entry 4i2x coloured by chain, side view.
PDB entry 4i2x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of signal regulatory protein gamma (SIRPγ) in complex with an antibody Fab fragment.
Nettleship JE, Ren J, Scott DJ, Rahman N, Hatherley D, Zhao Y, Stuart DI, Barclay AN, Owens RJ
BMC Struct Biol 13 13 (2013)
PMID: 23826770

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212806 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FabOX117 light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 214 amino acids
Theoretical weight: 23.66 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
FabOX117 heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 229 amino acids
Theoretical weight: 24.77 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Signal-regulatory protein gamma Chains: E, F
Molecule details ›
Chains: E, F
Length: 328 amino acids
Theoretical weight: 36.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9P1W8 (Residues: 29-347; Coverage: 89%)
Gene names: SIRPB2, SIRPG
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 2 x CL
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 140.361Å b: 174.191Å c: 81.734Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.26

Quick links

Citations

2 review citations

The interaction between signal regulatory protein alpha (SIRPα) and CD47: structure, function, and therapeutic target.
Barclay et al. (2014)
1 more

1 mention without citation

Crystal structure of signal regulatory protein gamma (SIRPγ) in complex with an antibody Fab fragment.
Nettleship et al. (2013)