› 4i9o

X-ray diffraction
2Å resolution

Crystal Structure of GACKIX L664C Tethered to 1-10

Released:
DOI: 10.2210/pdb4i9o/pdb
PDB entry 4i9o coloured by chain, front view.
PDB entry 4i9o coloured by chain, side view.
PDB entry 4i9o coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Ordering a dynamic protein via a small-molecule stabilizer.
Wang N, Majmudar CY, Pomerantz WC, Gagnon JK, Sadowsky JD, Meagher JL, Johnson TK, Stuckey JA, Brooks CL, Wells JA, Mapp AK
J Am Chem Soc 135 3363-6 (2013)
PMID: 23384013

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-155294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone lysine acetyltransferase CREBBP Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 12.14 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P45481 (Residues: 586-672; Coverage: 4%)
Gene names: Cbp, Crebbp
Sequence domains: KIX domain
Structure domains: Coactivator CBP, KIX domain

Ligands and Environments

2 bound ligands:
Ligand KI1 1 x KI1
Ligand EDO 2 x EDO
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P43212
Unit cell:
a: 48.33Å b: 48.33Å c: 85.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.233
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Small-molecule inhibitors of protein-protein interactions: progressing toward the reality.
Arkin et al. (2014)
9 more

8 mentions without citation

Modulating the masters: chemical tools to dissect CBP and p300 function.
Breen et al. (2018)
7 more