› 4iaq

X-ray diffraction
2.8Å resolution

Crystal structure of the chimeric protein of 5-HT1B-BRIL in complex with dihydroergotamine (PSI Community Target)

Released:
DOI: 10.2210/pdb4iaq/pdb
PDB entry 4iaq coloured by chain, front view.
PDB entry 4iaq coloured by chain, side view.
PDB entry 4iaq coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for molecular recognition at serotonin receptors.
Wang C, Jiang Y, Ma J, Wu H, Wacker D, Katritch V, Han GW, Liu W, Huang XP, Vardy E, McCorvy JD, Gao X, Zhou XE, Melcher K, Zhang C, Bai F, Yang H, Yang L, Jiang H, Roth BL, Cherezov V, Stevens RC, Xu HE
Science 340 610-4 (2013)
PMID: 23519210

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-hydroxytryptamine receptor 1B; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 45.52 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28222 (Residues: 33-239, 304-390; Coverage: 75%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: HTR1B, HTR1DB, cybC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 2GM 1 x 2GM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D, APS BEAMLINE 21-ID-D
Spacegroup: C222
Unit cell:
a: 84.74Å b: 192.579Å c: 74.341Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.236 0.257
Expression system: Spodoptera frugiperda

Quick links

Citations

72 review citations

DREADDs for Neuroscientists.
Roth BL. (2016)
71 more

49 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
48 more