› 4iax

X-ray diffraction
1.9Å resolution

Engineered human lipocalin 2 (CL31) in complex with Y-DTPA

Released:
DOI: 10.2210/pdb4iax/pdb
PDB entry 4iax coloured by chain, front view.
PDB entry 4iax coloured by chain, side view.
PDB entry 4iax coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-guided engineering of Anticalins with improved binding behavior and biochemical characteristics for application in radio-immuno imaging and/or therapy.
Eggenstein E, Eichinger A, Kim HJ, Skerra A
J Struct Biol 185 203-14 (2014)
PMID: 23542582

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil gelatinase-associated lipocalin Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 21.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P80188 (Residues: 21-198; Coverage: 100%)
Gene names: HNL, LCN2, NGAL
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand LIZ 1 x LIZ
Ligand YT3 1 x YT3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P41212
Unit cell:
a: 60.12Å b: 60.12Å c: 110.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.245
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Nanobodies and recombinant binders in cell biology.
Helma et al. (2015)
6 more

3 mentions without citation

Structural plasticity in the loop region of engineered lipocalins with novel ligand specificities, so-called Anticalins.
Achatz et al. (2022)
2 more