› 4ibw

X-ray diffraction
1.79Å resolution

Human p53 core domain with hot spot mutation R273H and second-site suppressor mutation T284R in sequence-specific complex with DNA

Released:
DOI: 10.2210/pdb4ibw/pdb
PDB entry 4ibw coloured by chain, front view.
PDB entry 4ibw coloured by chain, side view.
PDB entry 4ibw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural studies of p53 inactivation by DNA-contact mutations and its rescue by suppressor mutations via alternative protein-DNA interactions.
Eldar A, Rozenberg H, Diskin-Posner Y, Rohs R, Shakked Z
Nucleic Acids Res 41 8748-59 (2013)
PMID: 23863845

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117639 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA (5'-D(*CP*GP*GP*GP*CP*AP*TP*GP*CP*CP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 17 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 137.562Å b: 49.923Å c: 34.239Å
α: 90° β: 93.68° γ: 90°
R-values:
R R work R free
0.144 0.142 0.188
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

The p53 Pathway: Origins, Inactivation in Cancer, and Emerging Therapeutic Approaches.
Joerger et al. (2016)
7 more

3 mentions without citation

Intracellular pH Regulates Cancer and Stem Cell Behaviors: A Protein Dynamics Perspective.
Liu et al. (2020)
2 more