› 4ifb

X-ray diffraction
2.3Å resolution

Crystal structure of SULT 2A1 LLGG mutant with PAPS

Released:
DOI: 10.2210/pdb4ifb/pdb
PDB entry 4ifb coloured by chain, front view.
PDB entry 4ifb coloured by chain, side view.
PDB entry 4ifb coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kim J, Toro R, Bhosle R, Cook I, Wang T, Falany CN, Leyh TS, Almo SC

Function and Biology Details

Reactions catalysed: 
3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 2A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 33.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06520 (Residues: 1-285; Coverage: 100%)
Gene names: HST, STD, SULT2A1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand PO4 4 x PO4
Ligand PPS 2 x PPS
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 73.374Å b: 94.683Å c: 129.468Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.213
Expression system: Escherichia coli

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