› 4ifp

X-ray diffraction
1.99Å resolution

X-ray Crystal Structure of Human NLRP1 CARD Domain

Released:
DOI: 10.2210/pdb4ifp/pdb
PDB entry 4ifp coloured by chain, front view.
PDB entry 4ifp coloured by chain, side view.
PDB entry 4ifp coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the NLRP1 caspase recruitment domain suggests potential mechanisms for its association with procaspase-1.
Jin T, Curry J, Smith P, Jiang J, Xiao TS
Proteins 81 1266-70 (2013)
PMID: 23508996

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; NACHT, LRR and PYD domains-containing protein 1, C-terminus Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 466 amino acids
Theoretical weight: 51.7 KDa
Source organisms: Expression system: bacterium T452
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q9C000 (Residues: 1379-1462; Coverage: 6%)
Gene names: CARD7, DEFCAP, JW3994, KIAA0926, NAC, NALP1, NLRP1, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand MLI 3 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 187.011Å b: 108.772Å c: 121.266Å
α: 90° β: 120.98° γ: 90°
R-values:
R R work R free
0.172 0.17 0.208
Expression system: bacterium T452

Quick links

Citations

19 review citations

Regulation of inflammasome activation.
Man et al. (2015)
18 more

15 mentions without citation

Interferon-induced guanylate-binding proteins: Guardians of host defense in health and disease.
Tretina et al. (2019)
14 more