Function and Biology Details
Reaction catalysed:
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Ubiquitin and Ubiquitin-activating enzyme E1 1 (preferred)
PDBe Complex ID:
PDB-CPX-131719 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-activating enzyme E1 1
Molecule details ›
Chains: A, C
Length: 1001 amino acids
Theoretical weight: 111.76 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1001 amino acids
Theoretical weight: 111.76 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
O94609 (Residues: 13-1012; Coverage: 99%)
Sequence domains:
- ThiF family
- Ubiquitin-activating enzyme E1 FCCH domain
- Ubiquitin-activating enzyme E1 four-helix bundle
- Ubiquitin-activating enzyme, SCCH domain
- Ubiquitin fold domain
- Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1
- Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2
- Ubiquitin-activating enzyme E1, FCCH domain
- NAD(P)-binding Rossmann-like Domain
- Ubiquitin-activating enzyme E1, SCCH domain
- Ubiquitin-activating enzyme E1, UFD domain
Ubiquitin
Molecule details ›
Chains: B, D
Length: 96 amino acids
Theoretical weight: 10.74 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Length: 96 amino acids
Theoretical weight: 10.74 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0CH07 (Residues: 1-76; Coverage: 59%)
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
