› 4ima

X-ray diffraction
1.95Å resolution

The structure of C436M-hLPYK in complex with Citrate/Mn/ATP/Fru-1,6-BP

Released:
DOI: 10.2210/pdb4ima/pdb
PDB entry 4ima coloured by chain, front view.
PDB entry 4ima coloured by chain, side view.
PDB entry 4ima coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Energetic coupling between an oxidizable cysteine and the phosphorylatable N-terminus of human liver pyruvate kinase.
Holyoak T, Zhang B, Deng J, Tang Q, Prasannan CB, Fenton AW
Biochemistry 52 466-76 (2013)
PMID: 23270483

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKLR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 543 amino acids
Theoretical weight: 58.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30613 (Residues: 34-574; Coverage: 94%)
Gene names: PK1, PKL, PKLR
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MN 4 x MN
Ligand FLC 4 x FLC
Ligand FBP 4 x FBP
Ligand EDO 1 x EDO
Ligand ADN 1 x ADN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 82.751Å b: 204.727Å c: 86.532Å
α: 90° β: 96.75° γ: 90°
R-values:
R R work R free
0.2 0.198 0.233
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Nrf2 regulatory network provides an interface between redox and intermediary metabolism.
Hayes et al. (2014)
2 more

10 mentions without citation

Cross-link guided molecular modeling with ROSETTA.
Kahraman et al. (2013)
9 more