› 4irv

X-ray diffraction
2.04Å resolution

Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2

Released:
DOI: 10.2210/pdb4irv/pdb
PDB entry 4irv coloured by chain, front view.
PDB entry 4irv coloured by chain, side view.
PDB entry 4irv coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the Helicobacter pylori CagA oncoprotein bound to the human tumor suppressor ASPP2.
Nešić D, Buti L, Lu X, Stebbins CE
Proc Natl Acad Sci U S A 111 1562-7 (2014)
PMID: 24474782

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157375 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cytotoxicity-associated immunodominant antigen Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 221 amino acids
Theoretical weight: 25.48 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: P55980 (Residues: 19-235; Coverage: 18%)
Gene names: HP_0547, cag26, cagA, cai
Structure domains: Tetracycline Repressor; domain 2
Apoptosis-stimulating of p53 protein 2 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 62 amino acids
Theoretical weight: 6.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13625 (Residues: 726-782; Coverage: 5%)
Gene names: ASPP2, BBP, TP53BP2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 118.641Å b: 120.239Å c: 100.659Å
α: 90° β: 115.64° γ: 90°
R-values:
R R work R free
0.191 0.189 0.234
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Composition, structure and function of the Helicobacter pylori cag pathogenicity island encoded type IV secretion system.
Backert et al. (2015)
16 more

3 mentions without citation

Structure of the Helicobacter pylori CagA oncoprotein bound to the human tumor suppressor ASPP2.
Nešić et al. (2014)
2 more