› 4itj

X-ray diffraction
1.8Å resolution

Crystal structure of RIP1 kinase in complex with necrostatin-4

Released:
DOI: 10.2210/pdb4itj/pdb
PDB entry 4itj coloured by chain, front view.
PDB entry 4itj coloured by chain, side view.
PDB entry 4itj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of RIP1 inhibition by necrostatins.
Xie T, Peng W, Liu Y, Yan C, Maki J, Degterev A, Yuan J, Shi Y
Structure 21 493-9 (2013)
PMID: 23473668

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Receptor-interacting serine/threonine-protein kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 33.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13546 (Residues: 1-294; Coverage: 44%)
Gene names: RIP, RIP1, RIPK1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 1HX 2 x 1HX
Ligand IOD 9 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 46.841Å b: 97.136Å c: 128.119Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.222
Expression system: Spodoptera frugiperda

Quick links

Citations

53 review citations

Necroptosis.
Linkermann et al. (2014)
52 more

14 mentions without citation

When PERK inhibitors turn out to be new potent RIPK1 inhibitors: critical issues on the specificity and use of GSK2606414 and GSK2656157.
Rojas-Rivera et al. (2017)
13 more