› 4j38

X-ray diffraction
2.83Å resolution

Structure of Borrelia burgdorferi Outer surface protein E in complex with Factor H domains 19-20

Released:
DOI: 10.2210/pdb4j38/pdb
PDB entry 4j38 coloured by chain, front view.
PDB entry 4j38 coloured by chain, side view.
PDB entry 4j38 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for complement evasion by Lyme disease pathogen Borrelia burgdorferi.
Bhattacharjee A, Oeemig JS, Kolodziejczyk R, Meri T, Kajander T, Lehtinen MJ, Iwaï H, Jokiranta TS, Goldman A
J Biol Chem 288 18685-95 (2013)
PMID: 23658013

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140214 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Outer surface protein E Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 16.94 KDa
Source organism: Borreliella burgdorferi N40
Expression system: Escherichia coli
Structure domains: Borrelia outer surface protein E/F
Complement factor H Chain: B
Molecule details ›
Chain: B
Length: 129 amino acids
Theoretical weight: 14.61 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P08603 (Residues: 1103-1231; Coverage: 11%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)
Structure domains: Complement Module, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3121
Unit cell:
a: 86.405Å b: 86.405Å c: 107.784Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.255
Expression systems:
  • Escherichia coli
  • Komagataella pastoris

Quick links

Citations

14 review citations

Complement System Part II: Role in Immunity.
Merle et al. (2015)
13 more

8 mentions without citation

Complement Evasion by Lyme Disease Spirochetes.
Skare et al. (2020)
7 more