› 4j5l

X-ray diffraction
2.2Å resolution

Structure of the Cargo Binding Domain from Human Myosin Va

Released:
DOI: 10.2210/pdb4j5l/pdb
PDB entry 4j5l coloured by chain, front view.
PDB entry 4j5l coloured by chain, side view.
PDB entry 4j5l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into functional overlapping and differentiation among myosin V motors.
Nascimento AFZ, Trindade DM, Tonoli CCC, de Giuseppe PO, Assis LHP, Honorato RV, de Oliveira PSL, Mahajan P, Burgess-Brown NA, von Delft F, Larson RE, Murakami MT
J Biol Chem 288 34131-34145 (2013)
PMID: 24097982

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Unconventional myosin-Va Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 46.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y4I1 (Residues: 1448-1855; Coverage: 22%)
Gene names: MYH12, MYO5A
Sequence domains: DIL domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21
Unit cell:
a: 57.799Å b: 79.494Å c: 94.139Å
α: 90° β: 105.77° γ: 90°
R-values:
R R work R free
0.208 0.205 0.264
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Myosin-Driven Intracellular Transport.
Titus MA. (2018)
6 more

3 mentions without citation

Structural insights into functional overlapping and differentiation among myosin V motors.
Nascimento et al. (2013)
2 more