› 4j7y

X-ray diffraction
2.9Å resolution

Human LTC4 synthase in complex with product analogs - implications for enzyme catalysis

Released:
DOI: 10.2210/pdb4j7y/pdb
PDB entry 4j7y coloured by chain, front view.
PDB entry 4j7y coloured by chain, side view.
PDB entry 4j7y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.
Niegowski D, Kleinschmidt T, Olsson U, Ahmad S, Rinaldo-Matthis A, Haeggström JZ
J Biol Chem 289 5199-207 (2014)
PMID: 24366866

Function and Biology Details

Reaction catalysed: 
Leukotriene C(4) = leukotriene A(4) + glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-172606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leukotriene C4 synthase Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.41 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q16873 (Residues: 2-150; Coverage: 99%)
Gene name: LTC4S
Sequence domains: MAPEG family
Structure domains: Membrane associated eicosanoid/glutathione metabolism-like domain

Ligands and Environments


Cofactor: Ligand 1JP 1 x 1JP
2 bound ligands:
Ligand NI 1 x NI
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: F23
Unit cell:
a: 169.275Å b: 169.275Å c: 169.275Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.263
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Pichia pastoris as an expression host for membrane protein structural biology.
Byrne B. (2015)
4 more

2 mentions without citation

Structural basis for catalysis at the membrane-water interface.
Dufrisne et al. (2017)
1 more