› 4j8z

X-ray diffraction
2.42Å resolution

Crystal Structure of the Human SPOP BTB Domain

Released:
DOI: 10.2210/pdb4j8z/pdb
PDB entry 4j8z coloured by chain, front view.
PDB entry 4j8z coloured by chain, side view.
PDB entry 4j8z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of high-order oligomerization of the cullin-3 adaptor SPOP.
van Geersdaele LK, Stead MA, Harrison CM, Carr SB, Close HJ, Rosbrook GO, Connell SD, Wright SC
Acta Crystallogr D Biol Crystallogr 69 1677-84 (2013)
PMID: 23999291

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Speckle-type POZ protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43791 (Residues: 169-374; Coverage: 55%)
Gene name: SPOP
Sequence domains: BTB/POZ domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 111.39Å b: 71.58Å c: 62.22Å
α: 90° β: 117.21° γ: 90°
R-values:
R R work R free
0.209 0.207 0.255
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Targeting Cullin-RING E3 ubiquitin ligases for drug discovery: structure, assembly and small-molecule modulation.
Bulatov et al. (2015)
9 more

4 mentions without citation

RIT1 oncoproteins escape LZTR1-mediated proteolysis.
Castel et al. (2019)
3 more