› 4j9y

X-ray diffraction
1.51Å resolution

Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant

Released:
DOI: 10.2210/pdb4j9y/pdb
PDB entry 4j9y coloured by chain, front view.
PDB entry 4j9y coloured by chain, side view.
PDB entry 4j9y coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Unstructured to structured transition of an intrinsically disordered protein peptide in coupling Ca²⁺-sensing and SK channel activation.
Zhang M, Pascal JM, Zhang JF
Proc Natl Acad Sci U S A 110 4828-33 (2013)
PMID: 23487779

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144036 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Small conductance calcium-activated potassium channel protein 2 Chain: B
Molecule details ›
Chain: B
Length: 102 amino acids
Theoretical weight: 12.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P70604 (Residues: 396-487; Coverage: 16%)
Gene name: Kcnn2
Sequence domains: Calmodulin binding domain
Structure domains: Helix Hairpins
Calmodulin-1 Chain: R
Molecule details ›
Chain: R
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP29 (Residues: 1-149; Coverage: 100%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1
Sequence domains: EF-hand domain pair

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand CA 2 x CA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: C2
Unit cell:
a: 77.63Å b: 66.82Å c: 64.83Å
α: 90° β: 94.48° γ: 90°
R-values:
R R work R free
0.184 0.183 0.216
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Stabilization of protein-protein interactions in drug discovery.
Andrei et al. (2017)
13 more

5 mentions without citation

Unstructured to structured transition of an intrinsically disordered protein peptide in coupling Ca²⁺-sensing and SK channel activation.
Zhang et al. (2013)
4 more