› 4jbp

X-ray diffraction
2.45Å resolution

Novel Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules

Released:
DOI: 10.2210/pdb4jbp/pdb
PDB entry 4jbp coloured by chain, front view.
PDB entry 4jbp coloured by chain, side view.
PDB entry 4jbp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules.
Wu JM, Chen CT, Coumar MS, Lin WH, Chen ZJ, Hsu JT, Peng YH, Shiao HY, Lin WH, Chu CY, Wu JS, Lin CT, Chen CP, Hsueh CC, Chang KY, Kao LP, Huang CY, Chao YS, Wu SY, Hsieh HP, Chi YH
Proc Natl Acad Sci U S A 110 E1779-87 (2013)
PMID: 23610398

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aurora kinase A Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 32.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14965 (Residues: 123-401; Coverage: 69%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand YPH 1 x YPH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P6122
Unit cell:
a: 80.81Å b: 80.81Å c: 174.308Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.222 0.291
Expression system: Escherichia coli

Quick links

Citations

4 review citations

TPX2: of spindle assembly, DNA damage response, and cancer.
Neumayer et al. (2014)
3 more

2 mentions without citation

Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules.
Wu et al. (2013)
1 more