4jk6

X-ray diffraction
2.2Å resolution

Human urokinase-type Plasminogen Activator (uPA) in complex with a bicyclic peptide inhibitor (UK18-D-Aba)

Released:

Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-133264 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator chain B Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00749 (Residues: 179-423; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
bicyclic peptide UK18-D-Aba inhibitor of uPA Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 1.82 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: R3
Unit cell:
a: 121.219Å b: 121.219Å c: 42.731Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.153 0.205
Expression systems:
  • Homo sapiens
  • Not provided