› 4jkc

X-ray diffraction
1.5Å resolution

Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion

Released:
DOI: 10.2210/pdb4jkc/pdb
PDB entry 4jkc coloured by chain, front view.
PDB entry 4jkc coloured by chain, side view.
PDB entry 4jkc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.
Schellenberg MJ, Wu T, Ritchie DB, Fica S, Staley JP, Atta KA, LaPointe P, MacMillan AM
Nat Struct Mol Biol 20 728-34 (2013)
PMID: 23686287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-processing-splicing factor 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P2Q9 (Residues: 1769-1990; Coverage: 10%)
Gene names: PRPC8, PRPF8
Sequence domains: PRP8 domain IV core
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 4 x CL
Ligand GOL 2 x GOL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 76.195Å b: 77.932Å c: 93.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.128 0.164
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

The noncoding RNA revolution-trashing old rules to forge new ones.
Cech et al. (2014)
7 more

1 mention without citation

A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.
Schellenberg et al. (2013)