› 4jmg

X-ray diffraction
1.4Å resolution

Crystal structure of the synthetic protein in complex with pY peptide

Released:
DOI: 10.2210/pdb4jmg/pdb
PDB entry 4jmg coloured by chain, front view.
PDB entry 4jmg coloured by chain, side view.
PDB entry 4jmg coloured by chain, top view.
Source organisms:
Primary publication:
Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation.
Yasui N, Findlay GM, Gish GD, Hsiung MS, Huang J, Tucholska M, Taylor L, Smith L, Boldridge WC, Koide A, Pawson T, Koide S
Mol Cell 54 1034-41 (2014)
PMID: 24910098

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164633 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Clamp Ptpn11_pY580 Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.19 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Tyrosine-protein phosphatase non-receptor type 11 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06124 (Residues: 575-587; Coverage: 2%)
Gene names: PTP2C, PTPN11, SHPTP2

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 109.597Å b: 34.402Å c: 47.799Å
α: 90° β: 105.22° γ: 90°
R-values:
R R work R free
0.176 0.174 0.206
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Synthetic protein switches: design principles and applications.
Stein et al. (2015)
3 more

2 mentions without citation

Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation.
Yasui et al. (2014)
1 more