› 4jmh

X-ray diffraction
2.41Å resolution

Crystal structure of synthetic protein in complex with double pY peptide

Released:
DOI: 10.2210/pdb4jmh/pdb
PDB entry 4jmh coloured by chain, front view.
PDB entry 4jmh coloured by chain, side view.
PDB entry 4jmh coloured by chain, top view.
Source organisms:
Primary publication:
Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation.
Yasui N, Findlay GM, Gish GD, Hsiung MS, Huang J, Tucholska M, Taylor L, Smith L, Boldridge WC, Koide A, Pawson T, Koide S
Mol Cell 54 1034-41 (2014)
PMID: 24910098

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151420 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Clamp Shc1_pY239/240 Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 22.33 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Immunoglobulins
SHC-transforming protein 1 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29353 (Residues: 344-356; Coverage: 2%)
Gene names: SHC, SHC1, SHCA

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 89.853Å b: 92.19Å c: 59.596Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.276
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Monobodies and other synthetic binding proteins for expanding protein science.
Sha et al. (2017)
3 more

2 mentions without citation

Quercetin conjugated with superparamagnetic iron oxide nanoparticles improves learning and memory better than free quercetin via interacting with proteins involved in LTP.
Amanzadeh et al. (2019)
1 more