› 4jnw

X-ray diffraction
2.06Å resolution

Bacterially expressed Titin Kinase

Released:
DOI: 10.2210/pdb4jnw/pdb
PDB entry 4jnw coloured by chain, front view.
PDB entry 4jnw coloured by chain, side view.
PDB entry 4jnw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Titin kinase is an inactive pseudokinase scaffold that supports MuRF1 recruitment to the sarcomeric M-line.
Bogomolovas J, Gasch A, Simkovic F, Rigden DJ, Labeit S, Mayans O
Open Biol 4 140041 (2014)
PMID: 24850911

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Titin Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WZ42 (Residues: 32172-32492; Coverage: 1%)
Gene name: TTN
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 78.86Å b: 89.73Å c: 113.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.204
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Mechanotransduction in cardiac hypertrophy and failure.
Lyon et al. (2015)
15 more

2 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
1 more