› 4jo9

X-ray diffraction
2.5Å resolution

Crystal structure of the human Nup49CCS2+3* Nup57CCS3* complex 1:2 stoichiometry

Released:
DOI: 10.2210/pdb4jo9/pdb
PDB entry 4jo9 coloured by chain, front view.
PDB entry 4jo9 coloured by chain, side view.
PDB entry 4jo9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Architecture of the fungal nuclear pore inner ring complex.
Stuwe T, Bley CJ, Thierbach K, Petrovic S, Schilbach S, Mayo DJ, Perriches T, Rundlet EJ, Jeon YE, Collins LN, Huber FM, Lin DH, Paduch M, Koide A, Lu V, Fischer J, Hurt E, Koide S, Kossiakoff AA, Hoelz A
Science 350 56-64 (2015)
PMID: 26316600

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182244 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleoporin p54 Chains: A, C
Molecule details ›
Chains: A, C
Length: 40 amino acids
Theoretical weight: 4.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z3B4 (Residues: 453-491; Coverage: 8%)
Gene name: NUP54
Sequence domains: Nup54 C-terminal interacting domain
Nucleoporin p58/p45 Chain: B
Molecule details ›
Chain: B
Length: 87 amino acids
Theoretical weight: 10.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BVL2 (Residues: 341-426; Coverage: 14%)
Gene names: KIAA0410, NUP58, NUPL1
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P4122
Unit cell:
a: 55.483Å b: 55.483Å c: 191.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.246 0.294
Expression system: Escherichia coli

Quick links

Citations

22 review citations

The nuclear pore complex: understanding its function through structural insight.
Beck et al. (2017)
21 more

1 mention without citation

Architecture of the fungal nuclear pore inner ring complex.
Stuwe et al. (2015)