› 4jqi

X-ray diffraction
2.6Å resolution

Structure of active beta-arrestin1 bound to a G protein-coupled receptor phosphopeptide

Released:
DOI: 10.2210/pdb4jqi/pdb
PDB entry 4jqi coloured by chain, front view.
PDB entry 4jqi coloured by chain, side view.
PDB entry 4jqi coloured by chain, top view.
Source organisms:
Primary publication:
Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide.
Shukla AK, Manglik A, Kruse AC, Xiao K, Reis RI, Tseng WC, Staus DP, Hilger D, Uysal S, Huang LY, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ
Nature 497 137-41 (2013)
PMID: 23604254

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-210983 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-arrestin-1 Chain: A
Molecule details ›
Chain: A
Length: 401 amino acids
Theoretical weight: 45.06 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29066 (Residues: 2-393; Coverage: 94%)
Gene name: Arrb1
Sequence domains:
Structure domains:
Fab30 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab30 light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Vasopressin V2 receptor Chain: V
Molecule details ›
Chain: V
Length: 29 amino acids
Theoretical weight: 3.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P30518 (Residues: 343-371; Coverage: 8%)
Gene names: ADHR, AVPR2, DIR, DIR3, V2R

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand EDO 6 x EDO
Ligand PRO 1 x PRO
2 modified residues:
Ligand TPO 3 x TPO
Ligand SEP 5 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I212121
Unit cell:
a: 116.84Å b: 125.128Å c: 144.203Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.247
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

70 review citations

Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.
Artemenko et al. (2014)
69 more

69 mentions without citation

Structure and dynamics of GPCR signaling complexes.
Hilger et al. (2018)
68 more