› 4jrz

X-ray diffraction
2.4Å resolution

Human LTC4 synthase in complex with product analogs - implications for enzyme catalysis

Released:
DOI: 10.2210/pdb4jrz/pdb
PDB entry 4jrz coloured by chain, front view.
PDB entry 4jrz coloured by chain, side view.
PDB entry 4jrz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.
Niegowski D, Kleinschmidt T, Olsson U, Ahmad S, Rinaldo-Matthis A, Haeggström JZ
J Biol Chem 289 5199-207 (2014)
PMID: 24366866

Function and Biology Details

Reaction catalysed: 
Leukotriene C(4) = leukotriene A(4) + glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leukotriene C4 synthase Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.37 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q16873 (Residues: 2-150; Coverage: 99%)
Gene name: LTC4S
Sequence domains: MAPEG family
Structure domains: Membrane associated eicosanoid/glutathione metabolism-like domain

Ligands and Environments


Cofactor: Ligand GTX 1 x GTX
4 bound ligands:
Ligand NI 3 x NI
Ligand SO4 1 x SO4
Ligand PAM 1 x PAM
Ligand PLM 5 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: F23
Unit cell:
a: 169.108Å b: 169.108Å c: 169.108Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.282 0.233 0.264
Expression system: Komagataella pastoris

Quick links

Citations

6 review citations

Pichia pastoris as an expression host for membrane protein structural biology.
Byrne B. (2015)
5 more

1 mention without citation

Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.
Niegowski et al. (2014)