› 4jvg

X-ray diffraction
3.09Å resolution

B-Raf Kinase in Complex with Birb796

Released:
DOI: 10.2210/pdb4jvg/pdb
PDB entry 4jvg coloured by chain, front view.
PDB entry 4jvg coloured by chain, side view.
PDB entry 4jvg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibitors that stabilize a closed RAF kinase domain conformation induce dimerization.
Lavoie H, Thevakumaran N, Gavory G, Li JJ, Padeganeh A, Guiral S, Duchaine J, Mao DY, Bouvier M, Sicheri F, Therrien M
Nat Chem Biol 9 428-36 (2013)
PMID: 23685672

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 284 amino acids
Theoretical weight: 32.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15056 (Residues: 444-723; Coverage: 37%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand B96 4 x B96
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 54.997Å b: 120.61Å c: 81.714Å
α: 90° β: 90.44° γ: 90°
R-values:
R R work R free
0.235 0.232 0.295
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Targeting RAS-ERK signalling in cancer: promises and challenges.
Samatar et al. (2014)
21 more

6 mentions without citation

Inhibitors that stabilize a closed RAF kinase domain conformation induce dimerization.
Lavoie et al. (2013)
5 more