› 4jzn

X-ray diffraction
2.05Å resolution

Three dimensional structure of broadly neutralizing human anti - Hepatitis C virus (HCV) glycoprotein E2 Fab fragment HC84-1

Released:
DOI: 10.2210/pdb4jzn/pdb
PDB entry 4jzn coloured by chain, front view.
PDB entry 4jzn coloured by chain, side view.
PDB entry 4jzn coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of HCV neutralization by human monoclonal antibodies resistant to viral neutralization escape.
Krey T, Meola A, Keck ZY, Damier-Piolle L, Foung SK, Rey FA
PLoS Pathog 9 e1003364 (2013)
PMID: 23696737

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210941 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Anti-HCV E2 Fab HC84-1 heavy chain Chains: A, C, I
Molecule details ›
Chains: A, C, I
Length: 261 amino acids
Theoretical weight: 27.55 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
Structure domains: Immunoglobulins
Anti-HCV E2 Fab HC84-1 light chain Chains: B, D, P
Molecule details ›
Chains: B, D, P
Length: 215 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
Structure domains: Immunoglobulins
Envelope glycoprotein E2 Chain: K
Molecule details ›
Chain: K
Length: 13 amino acids
Theoretical weight: 1.54 KDa
Source organism: Hepatitis C virus (isolate H)
Expression system: Not provided
UniProt:
  • Canonical: P27958 (Residues: 434-446; Coverage: 0%)

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 76.521Å b: 165.568Å c: 269.323Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.235
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

19 review citations

Peptide-Based Vaccines: Current Progress and Future Challenges.
Malonis et al. (2020)
18 more

13 mentions without citation

VH1-69 antiviral broadly neutralizing antibodies: genetics, structures, and relevance to rational vaccine design.
Chen et al. (2019)
12 more