› 4k0v

X-ray diffraction
4.51Å resolution

Structural basis for angiopoietin-1 mediated signaling initiation

Released:
DOI: 10.2210/pdb4k0v/pdb
PDB entry 4k0v coloured by chain, front view.
PDB entry 4k0v coloured by chain, side view.
PDB entry 4k0v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for angiopoietin-1-mediated signaling initiation.
Yu X, Seegar TC, Dalton AC, Tzvetkova-Robev D, Goldgur Y, Rajashankar KR, Nikolov DB, Barton WA
Proc Natl Acad Sci U S A 110 7205-10 (2013)
PMID: 23592718

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169750 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Angiopoietin-1 receptor Chain: A
Molecule details ›
Chain: A
Length: 529 amino acids
Theoretical weight: 58.9 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02763 (Residues: 23-542; Coverage: 47%)
Gene names: TEK, TIE2, VMCM, VMCM1
Sequence domains:
Angiopoietin-1 Chain: B
Molecule details ›
Chain: B
Length: 230 amino acids
Theoretical weight: 26.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15389 (Residues: 280-498; Coverage: 45%)
Gene names: ANGPT1, KIAA0003
Sequence domains: Fibrinogen beta and gamma chains, C-terminal globular domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I4122
Unit cell:
a: 189.533Å b: 189.533Å c: 334.867Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.248 0.288
Expression system: Spodoptera frugiperda

Quick links

Citations

14 review citations

Therapeutic targeting of the angiopoietin-TIE pathway.
Saharinen et al. (2017)
13 more

10 mentions without citation

Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization.
Leppänen et al. (2017)
9 more