› 4k2r

X-ray diffraction
3Å resolution

Structural basis for activation of ZAP-70 by phosphorylation of the SH2-kinase linker

Released:
DOI: 10.2210/pdb4k2r/pdb
PDB entry 4k2r coloured by chain, front view.
PDB entry 4k2r coloured by chain, side view.
PDB entry 4k2r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for activation of ZAP-70 by phosphorylation of the SH2-kinase linker.
Yan Q, Barros T, Visperas PR, Deindl S, Kadlecek TA, Weiss A, Kuriyan J
Mol Cell Biol 33 2188-201 (2013)
PMID: 23530057

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ZAP-70 Chain: A
Molecule details ›
Chain: A
Length: 614 amino acids
Theoretical weight: 69.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43403 (Residues: 1-606; Coverage: 98%)
Gene names: SRK, ZAP70
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 48.53Å b: 53.15Å c: 68.96Å
α: 106.1° β: 93.26° γ: 104.4°
R-values:
R R work R free
0.213 0.209 0.287
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

TCR Signaling: Mechanisms of Initiation and Propagation.
Courtney et al. (2018)
14 more

14 mentions without citation

Immune Checkpoint Receptors Signaling in T Cells.
Baldanzi G. (2022)
13 more